Our scientific team led by Dr. Enrico Pierluigi Spugnini and Dr. Alfonso Baldi has published in the last 10 years many articles on Electroporation and Electrochemotherapy. These studies have allowed us to design and improve the electroporator Onkodisruptor®.

Electroporation Publications List 2003 – 2019

Electroporation Publications

  • Spugnini EP, A Porrello. Potentiation of chemotherapy in companion animals with spontaneous large neoplasms by application of biphasic electric pulses. J. Exp. Clin. Canc. Res. 2003; 22: 571-580.
  • Spugnini EP, Dragonetti E, Vincenzi B, Onori E, Citro G, Baldi A. Pulse mediated chemotherapy enhances local control and survival in a spontaneous canine mucosal melanoma model. Melanoma Res. 2006; 16: 23-27.
  • Spugnini EP, Baldi A, Vincenzi B, Bongiorni F, Bellelli C, Citro G, Porrello A. Intraoperative versus postoperative electrochemotherapy in soft tissue sarcomas: a preliminary study in a spontaneous feline model. Cancer Chemother Pharmacol. 2007; 59 (3): 375-381.
  • Spugnini EP, Vincenzi B, Baldi F, Citro G, Baldi A. Adjuvant electrochemotherapy for the treatment of incompletely resected canine mast cell tumors. Anticancer Res. 2006; 26(6B) 4585-4889.
  • Spugnini EP, Vincenzi B, Citro G, Santini D, Dotsinsky I, Mudrov N, Montesarchio V, Laieta MT, Esposito V,Baldi A . Adjuvant electrochemotherapy for the treatment of incompletely excised spontaneous canine sarcomas. In Vivo 2007; 21: 819-822.
  • Spugnini EP, Filipponi M, Romani L, Dotsinsky I, Mudrov N, Baroni A, Ruocco E, Lieta MT, Montesarchio V,Cassandro R, Citro G, Baldi A. Local control and distant metastases after electrochemotherapy of a canine anal melanoma. In Vivo 2007; 21: 897-900.
  • Spugnini EP, Citro G, Mellone P, Dotsinsky I, Mudrov N, Baldi A. Electrochemotherapy for localized lymphoma: a preliminary study in companion animals. J Exp Clin Cancer Res 2007; 26: 343-346.
  • Spugnini EP, Arancia G, Porrello A, Colone M, Formisano G, Stringaro A, Citro G, Molinari A. Ultrastructural modifications induced by electroporation on melanoma xenografts. Micr Res Tech.2007; 70: 1041-1050.
  • Spugnini EP, Citro G, Dotsinsky I, Mudrov N, Mellone P, Baldi A. Ganglioneuroblastoma in a cat: a rare neoplasm treated with electrochemotherapy. Vet J 2008; 178: 291-293.
  • Spugnini EP, Vincenzi B, Citro G, Tonini G, Dotsinsky I, Mudrov N, Baldi A. Electrochemotherapy for the treatment of squamous cell carcinoma in cats: a preliminary report. Vet J 2009; 179: 117-120.
  • Spugnini EP, Vincenzi B, Betti G, Cordahi F, Dotsinsky I, Mudrov N, Citro G, Baldi A. Surgery and electrochemotherapy for high grade soft tissue sarcoma in a dog. Vet Rec 2008; 162: 186-188.
  • Spugnini EP, Dotsinsky I, Mudrov N, Cardosi G, Citro G, Baldi A. Biphasic pulses enhance bleomycin efficacy in a spontaneous canine perianal tumors model. J Exp Clin Cancer Res 2007; 26: 483-487.
  • Spugnini EP, Dotsinsky I, Mudrov N, Bufalini M, Giannini G, Citro G, Feroce F, Baldi A. Adjuvant electrochemotherapy for incompletely excised anal sac carcinoma in a dog. In Vivo 2008; 22:. 47-50
  • Spugnini EP, Dotsinsky I, Mudrov N, De Luca A, Codini C, Citro G, D’ Avino A, Baldi A. Successful rescue of an apocrine gland carcinoma metastatic to the cervical lymph nodes by mitoxantrone coupled with trains of permeabilizing electric pulses (electrochemotherapy). In Vivo 2008; 22: 51-54
  • Spugnini EP, Filipponi M, Romani L, Dotsinsky I, Mudrov N, Citro G, Baldi A. Bilateral pleomorphic rhabdomyosarcoma in a cat treated with electrochemotherapy. J Small An Pract 2010; 51(6):330-2
  • Spugnini EP, Vincenzi B, Citro G, Baldi A. Evaluation of cisplatin (CDDP) as a electrochemotherapy agent for the treatment of incompletely excised mast cell tumors in dogs. J Vet Intern Med, 2011; 25: 407-411.
  • Spugnini EP, Biroccio A, De Mori R, Scarsella M, D’ Angelo C, Baldi A, Leonetti C. Electroporation increases antitumoral efficacy of the bcl-2 antisense g3139 and chemotherapy in a human melanoma xenograft. J Trasl Med. 2011;9:152.
  • Spugnini EP, D’ Alterio GL, Dotsinsky I, Mudrov T, Dragonetti E, Murace G, Citro G, Baldi A. Electrochemotherapy for the treatment of multiple melanomas in a horse. J Eq Vet Sci. 2011; 31: 430-433.
  • Spugnini EP, Renaud SM, Buglioni S, Carocci F, Dragonetti E, Murace R, Cardelli P, Vincenzi B, Baldi A, CitroG. Electrochemotherapy with cisplatin enhances local control after surgical ablation of fibrosarcoma in cats: an approach to improve the therapeutic index of highly toxic chemotherapy drugs. J Transl Med. 2011; 9:152.
  • Spugnini EP, Cardillo I, Fanciulli M, Crispi S, Baldi A. Electroporation as a strategy to promote HtrA1 gene uptake and chemotherapy efficacy in a mouse model of mesothelioma. Front Biosci 2013; 5:974-81.
  • Spugnini EP, Di Tosto G, Salemme S, Pecchia L, Fanciulli M, Baldi A. Electrochemotherapy for the treatment of recurring aponeurotic fibromatosis in a dog. Can Vet J 2013; 54: 606-608.
  • Scacco L, Bolaffio C, Romano A, Fanciulli M, Baldi A, Spugnini EP. Adjuvant electrochemotherapy increases local control in a recurring equine anal melanoma. J Eq Vet Sci. 2013; 33: 637-639.
  • Spugnini EP, Melillo A, Quagliuolo L, Boccellino M, Vincenzi B, Pasquali P, Baldi A. Definition of novel electrochemotherapy parameters and validation of their in vitro and in vivo effectiveness. J Cell Physiol. 2014 Sep;229(9):1177-81. doi: 10.1002/jcp.24548. PubMed PMID: 24403005.
  • Spugnini EP, Baldi A. Electrochemotherapy in veterinary oncology: from rescue to first line therapy. Methods Mol Biol. 2014;1121:247-56.doi:10.1007/978-1-4614-9632-8_22. Review. PubMed PMID: 24510829.
  • Lanza A, Baldi A, Spugnini EP. Surgery and electrochemotherapy for the treatment of cutaneous squamous cell carcinoma in a yellow-bellied slider (Trachemys scripta scripta). J Am Vet Med Assoc. 2015 Feb 15;246(4):455-7. doi:10.2460/javma.246.4.455. PubMed PMID: 25632821.
  • Spugnini EP,M Pizzuto,M Filipponi,L Romani, B Vincenzi,F Menicagli. Electroporation Enhances Bleomycin Efficacy in Cats with Periocular Carcinoma and Advanced Squamous Cell Carcinoma of the Head – Journal of Veterinary Internal Medicine(Impact Factor: 1.88)
  • Spugnini, E.P., Bolaffio, C., Scacco, I. and Baldi, A. 2016. Electrochemotherapy increases local control after incomplete excision of a recurring penile fibrosarcoma in a stallion. Open Vet. J. 6(3), 234-237.
  • Lanza A, Pettorali M, Baldi A, Spugnini EP. Surgery and electrochemotherapy treatment of incompletely excised mammary carcinoma in two male pet rats(Rattus norvegicus). J. Vet. Med. Sci. In press (2017)
  • Spugnini EP, Menicagli F, Pettorali M, Baldi A. Ultrasound guided electrochemotherapy for the treatment of a clear cell thymoma in a cat. Open Vet j. 2017;7(1):57-60. doi: 10.4314/ovj.v7i1.8. Epub 2017 Mar 8. PubMed PMID: 28331834; PubMed Central PMCID: PMC5356291.
  • Spugnini EP, Bolaffio C, Scacco L, Baldi A. Isolated limb perfusion electrochemotherapy for the treatment of an advanced squamous cell carcinoma of the hoof in a mare. Open Vet J. 2017;7(2):192-196. doi: 10.4314/ovj.v7i2.18. Epub 2017 Jun 28. PubMed PMID: 28717603; PubMed Central PMCID: PMC5498771.
  • Pasquali P, Freites-Martinez A, Gonzalez S, Spugnini EP, Baldi A. Successful treatment of plantar warts with intralesional bleomycin and electroporation: pilot prospective study. Dermatol Pract Concept. 2017 Jul 31;7(3):21-26. doi: 10.5826/dpc.0703a04. eCollection 2017 Jul. PubMed PMID: 29085716; PubMed Central  PMCID: PMC5661162.
  • Pasquali P, Spugnini EP, Baldi A. Successful Treatment of a Keratoacanthoma with Electrochemotherapy: A Case Report. Dermatol Ther (Heidelb). 2018 Mar;8(1):143-146. doi: 10.1007/s13555-018-0222-9. Epub 2018 Jan 24. PubMed PMID: 29368221; PubMed Central PMCID: PMC5825327.
  • Spugnini EP, Lanza A., Sebasti S., Baldi A., Electrochemotherapy palliation of an oral squamous cell carcinoma in an African hedgehog (Atelerix albiventris), Epub Autumn 2018, Veterinary Research Forum. 2018; 9 (4) 379 – 381 doi: 10.30466/vrf.2018.33109.
  • Enrico P. Spugnini, Bruno Vincenzi, Bruno Amadio, Alfonso Baldi, Adjuvant electrochemotherapy with bleomycin and cisplatin combination for canine soft tissue sarcomas: A study of 30 cases, Open Veterinary Journal, (2019), Vol. 9(1): 88–93, Published: 25/03/2019, https://www.ajol.info/index.php/ovj/article/view/185281
  • From the prestigious oncology magazine Clin Cancer Res: OnkoDisruptor® was used to carry the MC2494 molecule:
  • RIP1-HAT1-SIRT Complex Identification and Targeting in Treatment and Prevention of Cancer. Clin Cancer Res. 2018 Jun 15;24(12):2886-2900. doi:       10.1158/1078-0432.CCR-17-3081. Epub 2018 Mar 13. https://www.ncbi.nlm.nih.gov/pubmed/?term=RIP1-HAT1- SirT+complex+identification+and+targeting+in+treatment+and+prevention+of+cancer
  • Spugnini EP, Baldi A, Electrochemotherapy in Veterinary Oncology: State-of-the-Art and Perspectives, Vet Clin North Am Small Anim Pract. 2019 Sep;49(5):967-979. doi: 10.1016/j.cvsm.2019.04.006. Epub 2019 Jun 6, Epub “Cancer in companions animals”, Sep. 2019,
  • Lanza A., Baldi A., Rossi G., Spugnini EP., Electrochemotherapy for the treatment of an incompletely excised subcutaneous low-grade epithelioid hemangioendothelioma in a budgerigar parakeet (Melopsittacus undulatus), Open Veterinary Journal, (2019), Vol. 9(3): 269–272, DOI: http://dx.doi.org/10.4314/ovj.v9i3.13, Epub September 2019.
ElectroporatorOnkodisruptorcancer chemotherapy and pharmacology Cancer
Electroporator Onkodisruptor journal internal vet
Electroporator Onkodisruptor JEVS logo
Electroporator Onkodisruptor in vivo
Electroporator Onkodisruptor pubmedlogo
Electroporator Onkodisruptor translmedlogo